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primary antibodies against β actin  (ABclonal Biotechnology)


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    Structured Review

    ABclonal Biotechnology primary antibodies against β actin
    Primary Antibodies Against β Actin, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 2395 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+actb+(actin+beta)/ACTB+Rabbit+mAb/pm42302427-64-0-28
    Average 99 stars, based on 2395 article reviews
    primary antibodies against β actin - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    other:

    Article Title: Intratracheal Man-Rg3-Lipo Attenuates OLV-Induced Lung Injury in Juvenile Rats and Is Associated with Macrophage-Related NF-κB Suppression.
    Article Snippet: Introduction: To determine whether intratracheally administered mannose-modified ginsenoside Rg3 liposomes (Man-Rg3-Lipo) mitigate One-Lung Ventilation (OLV)-induced lung injury in juvenile rats and to explore the associated pulmonary distribution, macrophage-associated inflammatory regulation, and NF-κB signaling changes.. Methods: Man-Rg3-Lipo was prepared by thin-film dispersion and characterized for morphology, particle size, Polydispersity Index (PDI), zeta potential, Encapsulation Efficiency (EE), Drug Loading (DL), and in vitro release.. Ex vivo fluorescence imaging was performed after intratracheal administration to assess pulmonary distribution.

    Article Title: WTAP Transcriptional Suppression by KLF9 Drives Osteoclastogenesis via M 6 A‐Mediated Regulation of CSF1R Signaling in Estrogen‐Deficient Osteoporosis
    Article Snippet: Proteins were extracted from BMDMs using RIPA lysis buffer (Beyotime, Shanghai, China) containing protease and phosphatase inhibitors.

    Article Title: Characterization and comparison of the structure and antioxidant activity of queen bee larvae (Apis mellifera) hydrolysates processed with different proteases.
    Article Snippet: Queen bee larvae (QBL), a by-product of royal jelly processing, represent a high-quality protein source that has not been fully utilized.. In this study, the structural characteristics and antioxidant activities of QBL hydrolysates (QBLHs) produced using eight distinct proteases were investigated.. The results showed that enzymatic hydrolysis altered the secondary and tertiary structure of QBL proteins and resulted in a looser and more irregular protein structure.

    Membrane:

    Article Title: LIMA1/EZR axis promotes MASH progression by reducing chaperone-mediated autophagy dependent-lipophagy in hepatocytes
    Article Snippet: .. Next, the membrane was blocked with 5% milk at room temperature for 1 h. The membrane with blotted protein was incubated with primary antibodies against β-actin (AC006, Abclonal, Wuhan, China), LIMA1 (A11682, Abclonal), LC3A/LC3B (A5618, Abclonal), SQSTM1 (18420-1-AP, Proteintech, Wuhan, China), LAMP1 (21997-1-AP, Proteintech), ATG5/ATG12 (sc-133158, Santa Cruz, Dallas, TX, USA), ATGL (AFRM82338, AiFang, Changsha, China), LAMP2a (AFRM80516, AiFang), HSC70 (AF11309, AiFang), PLIN2 (AF10490, AiFang), p-EZR (AP0207, Abclonal), EZR (A0703, Abclonal), ROCK1 (21850-1-AP, Proteintech), ALDH1A1 (AFRM9438, AiFang), pAKT (66444-1-Ig, Proteintech), AKT (60203-2-Ig, Proteintech), p-IRS1 (44818G, Invitrogen), IRS1 (PA1-1059, Invitrogen), His tag (AE086, Abclonal), GST tag (10000- 0-AP, Proteintech), DYKDDDDK tag (66008-4-Ig, Proteintech), MYC tag (60003-2- Ig, Proteintech), and α-SMA (55135-1-AP, Proteintech). .. Following this, the membrane was probed with horseradish peroxidase-conjugated secondary antibodies (31460, Invitrogen), and the protein bands were visualized using enhanced chemiluminescence reagents (E412-01, Vazyme, Nanjing, China).

    Incubation:

    Article Title: LIMA1/EZR axis promotes MASH progression by reducing chaperone-mediated autophagy dependent-lipophagy in hepatocytes
    Article Snippet: .. Next, the membrane was blocked with 5% milk at room temperature for 1 h. The membrane with blotted protein was incubated with primary antibodies against β-actin (AC006, Abclonal, Wuhan, China), LIMA1 (A11682, Abclonal), LC3A/LC3B (A5618, Abclonal), SQSTM1 (18420-1-AP, Proteintech, Wuhan, China), LAMP1 (21997-1-AP, Proteintech), ATG5/ATG12 (sc-133158, Santa Cruz, Dallas, TX, USA), ATGL (AFRM82338, AiFang, Changsha, China), LAMP2a (AFRM80516, AiFang), HSC70 (AF11309, AiFang), PLIN2 (AF10490, AiFang), p-EZR (AP0207, Abclonal), EZR (A0703, Abclonal), ROCK1 (21850-1-AP, Proteintech), ALDH1A1 (AFRM9438, AiFang), pAKT (66444-1-Ig, Proteintech), AKT (60203-2-Ig, Proteintech), p-IRS1 (44818G, Invitrogen), IRS1 (PA1-1059, Invitrogen), His tag (AE086, Abclonal), GST tag (10000- 0-AP, Proteintech), DYKDDDDK tag (66008-4-Ig, Proteintech), MYC tag (60003-2- Ig, Proteintech), and α-SMA (55135-1-AP, Proteintech). .. Following this, the membrane was probed with horseradish peroxidase-conjugated secondary antibodies (31460, Invitrogen), and the protein bands were visualized using enhanced chemiluminescence reagents (E412-01, Vazyme, Nanjing, China).

    Article Title: Engineered Lactococcus lactis for Oral Delivery of an Antimicrobial Peptide against Enterotoxigenic Escherichia coli in Weaned Piglets.
    Article Snippet: Food-grade strategies against foodborne pathogens while preserving intestinal health are gaining attention.. Here, we developed a regulated delivery platform using Lactococcus lactis engineered to secrete a tandem dimeric antimicrobial peptide (SD).. The engineered strain (SDLactis) exhibited stable growth, genetic stability, and tolerance to simulated gastrointestinal conditions.

    Article Title: Enzyme-Responsive Composite Hydrogel Coating Containing Nano-HA/Nano-ZnO with Pro-Osteogenic and Anti-Osteoclastic Properties.
    Article Snippet: The treatment of osteoporotic bone defects remains challenging due to the pathological microenvironment with impaired regenerative capacity and imbalanced bone formation and resorption.. In this study, a methacrylated gelatin (GelMA) and methacrylated chitosan (CSMA) composite hydrogel coating is developed on poly(phthalazinone ether nitrile ketone) (PPENK), showing pro-osteogenic and anti-osteoclastic effects in vitro.. Nano-zinc oxide (nano-ZnO), nano-hydroxyapatite (nano-HA), and phosphate-ester-functionalized alendronate (ALNP) are incorporated to confer antibacterial activity, promote osteogenesis, and enable enzyme-responsive release of anti-osteoporosis alendronate (ALN).

    Article Title: Integrated proteomics and metabolomics reveal mechanisms of blood pressure reduction in spontaneously hypertensive rats under hypoxic conditions
    Article Snippet: .. After transfer, membranes were blocked with 5% non-fat milk at room temperature for 2 h, followed by 12–16 h of incubation at 4 °C with primary antibodies against β-actin (1:50000, Abclonal, AC026), AcsI1 (1:2000, Huabio, ER60807), Cox5a (1:2000, Abclonal, A6437), Cpt2 (1:1000, Huabio, ET1611-64), Kng1 (1:1000, Abclonal, A1670), Ndufs1 (1:2000, Abclonal, A21192), and Uqcrc2 (1:2000, Proteinetch, 14742-1-AP). ..

    Western Blot:

    Article Title: Enzyme-Responsive Composite Hydrogel Coating Containing Nano-HA/Nano-ZnO with Pro-Osteogenic and Anti-Osteoclastic Properties.
    Article Snippet: The treatment of osteoporotic bone defects remains challenging due to the pathological microenvironment with impaired regenerative capacity and imbalanced bone formation and resorption.. In this study, a methacrylated gelatin (GelMA) and methacrylated chitosan (CSMA) composite hydrogel coating is developed on poly(phthalazinone ether nitrile ketone) (PPENK), showing pro-osteogenic and anti-osteoclastic effects in vitro.. Nano-zinc oxide (nano-ZnO), nano-hydroxyapatite (nano-HA), and phosphate-ester-functionalized alendronate (ALNP) are incorporated to confer antibacterial activity, promote osteogenesis, and enable enzyme-responsive release of anti-osteoporosis alendronate (ALN).

    Blocking Assay:

    Article Title: Enzyme-Responsive Composite Hydrogel Coating Containing Nano-HA/Nano-ZnO with Pro-Osteogenic and Anti-Osteoclastic Properties.
    Article Snippet: The treatment of osteoporotic bone defects remains challenging due to the pathological microenvironment with impaired regenerative capacity and imbalanced bone formation and resorption.. In this study, a methacrylated gelatin (GelMA) and methacrylated chitosan (CSMA) composite hydrogel coating is developed on poly(phthalazinone ether nitrile ketone) (PPENK), showing pro-osteogenic and anti-osteoclastic effects in vitro.. Nano-zinc oxide (nano-ZnO), nano-hydroxyapatite (nano-HA), and phosphate-ester-functionalized alendronate (ALNP) are incorporated to confer antibacterial activity, promote osteogenesis, and enable enzyme-responsive release of anti-osteoporosis alendronate (ALN).



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    Cell Signaling Technology Inc primary antibodies against human actb
    Fig. 7. SP1 regulates SLC7A3 expression. A-B. mRNA expression (A) and protein level (B) of SLC7A3 in OS cells with SP1 knockdown or overexpression (n = 3). C. Arginine uptake ratio in OS cells with/without SP1 knockdown upon SLC7A3 overexpression (n = 3). D. Schematic diagram showing possible regulatory sites for SP1 in the SLC7A3 promoter predicted by the JASPAR database (top). Construction of the SLC7A3 luciferase plasmids: wild-type SLC7A3 promoter (WT) and deletion mutants lacking region 1 (ΔRegion 1), region 2 (ΔRegion 2), or both (ΔRegion 1 + 2) (bottom). E. Homology analysis of region 1 and region 2 in the SLC7A3 promoter. F. 143B cells with/without SIRPA knockdown were transfected with plasmids encoding SP1(-500/0)-Luc WT, ΔRegion1, ΔRegion2, and ΔRegion1+2. Then, luciferase levels were measured in extracts after 48 h. G-H. ChIP assay of DNA fragments containing putative region 1 and region 2 within the SLC7A3 promoter region followed by PCR. The SLC7A3 promotor region beyond SP1 regulation (CTR) and the β-actin region <t>(ACTB)</t> were used as negative controls; an anti- histone antibody and rabbit IgG were used as assay controls (G). The histogram shows the relative amount of each DNA fragment binding to SP1 (H). The data are presented as the means±SDs. *P < 0.05, **P < 0.01 by one-way ANOVA.
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    Image Search Results


    Journal: Frontiers in Physiology

    Article Title: Transcriptomic analysis and experimental verification reveal the involvement of PI3K/AKT signaling pathway in high-altitude cognitive dysfunction

    doi: 10.3389/fphys.2026.1781613

    Figure Lengend Snippet: Primer sequences for the genes observed in qRT-PCR.

    Article Snippet: Primary antibody against β -actin were purchased from Zhongshan Golden Bridge Biological Technology Co., Ltd. (Beijing, China).

    Techniques:

    Effects of HH on the BBB integrity in mouse hippocampus. (A) Representative images of EB extravasation in the hippocampus. (B) Quantitative analysis of EB leakage. (C) Representative western blots of ZO-1 and occludin. Quantitative analysis of the ZO-1/β-actin (D) and occludin/β-actin (E) ratios. Data are presented as mean ± SD. Statistical comparisons were performed by Student’s t test. For EB, n = 6 per group; for Western blot analysis, n = 3 per group, ** p < 0.01 vs. Con group.

    Journal: Frontiers in Physiology

    Article Title: Transcriptomic analysis and experimental verification reveal the involvement of PI3K/AKT signaling pathway in high-altitude cognitive dysfunction

    doi: 10.3389/fphys.2026.1781613

    Figure Lengend Snippet: Effects of HH on the BBB integrity in mouse hippocampus. (A) Representative images of EB extravasation in the hippocampus. (B) Quantitative analysis of EB leakage. (C) Representative western blots of ZO-1 and occludin. Quantitative analysis of the ZO-1/β-actin (D) and occludin/β-actin (E) ratios. Data are presented as mean ± SD. Statistical comparisons were performed by Student’s t test. For EB, n = 6 per group; for Western blot analysis, n = 3 per group, ** p < 0.01 vs. Con group.

    Article Snippet: Primary antibody against β -actin were purchased from Zhongshan Golden Bridge Biological Technology Co., Ltd. (Beijing, China).

    Techniques: Western Blot

    Effects of HH on the expression of genes and proteins related to PI3K/AKT signaling pathway in mouse hippocampus. (A) The relative mRNA expression levels of Kdr , Spp1 , Vwf , and Vegfa screened via qRT-PCR. (B) Representative western blots of p-PI3K, PI3K, p-AKT, and AKT. Quantitative analysis of the p-PI3K/PI3K (C) and p-AKT/AKT (D) ratios. (E) Representative western blots of Nrf2, HO-1, p-NF-κB, and NF-κB. Quantitative analysis of the Nrf2/β-actin (F) , HO-1/β-actin (G) , and p-NF-κB/NF-κB (H) ratios. Data are presented as mean ± SD. Statistical comparisons were performed by Student’s t test. For qRT-PCR, n = 6 per group; for Western blot analysis, n = 3 per group, * p < 0.05, ** p < 0.01 vs. Con group.

    Journal: Frontiers in Physiology

    Article Title: Transcriptomic analysis and experimental verification reveal the involvement of PI3K/AKT signaling pathway in high-altitude cognitive dysfunction

    doi: 10.3389/fphys.2026.1781613

    Figure Lengend Snippet: Effects of HH on the expression of genes and proteins related to PI3K/AKT signaling pathway in mouse hippocampus. (A) The relative mRNA expression levels of Kdr , Spp1 , Vwf , and Vegfa screened via qRT-PCR. (B) Representative western blots of p-PI3K, PI3K, p-AKT, and AKT. Quantitative analysis of the p-PI3K/PI3K (C) and p-AKT/AKT (D) ratios. (E) Representative western blots of Nrf2, HO-1, p-NF-κB, and NF-κB. Quantitative analysis of the Nrf2/β-actin (F) , HO-1/β-actin (G) , and p-NF-κB/NF-κB (H) ratios. Data are presented as mean ± SD. Statistical comparisons were performed by Student’s t test. For qRT-PCR, n = 6 per group; for Western blot analysis, n = 3 per group, * p < 0.05, ** p < 0.01 vs. Con group.

    Article Snippet: Primary antibody against β -actin were purchased from Zhongshan Golden Bridge Biological Technology Co., Ltd. (Beijing, China).

    Techniques: Expressing, Quantitative RT-PCR, Western Blot

    Fig. 7. SP1 regulates SLC7A3 expression. A-B. mRNA expression (A) and protein level (B) of SLC7A3 in OS cells with SP1 knockdown or overexpression (n = 3). C. Arginine uptake ratio in OS cells with/without SP1 knockdown upon SLC7A3 overexpression (n = 3). D. Schematic diagram showing possible regulatory sites for SP1 in the SLC7A3 promoter predicted by the JASPAR database (top). Construction of the SLC7A3 luciferase plasmids: wild-type SLC7A3 promoter (WT) and deletion mutants lacking region 1 (ΔRegion 1), region 2 (ΔRegion 2), or both (ΔRegion 1 + 2) (bottom). E. Homology analysis of region 1 and region 2 in the SLC7A3 promoter. F. 143B cells with/without SIRPA knockdown were transfected with plasmids encoding SP1(-500/0)-Luc WT, ΔRegion1, ΔRegion2, and ΔRegion1+2. Then, luciferase levels were measured in extracts after 48 h. G-H. ChIP assay of DNA fragments containing putative region 1 and region 2 within the SLC7A3 promoter region followed by PCR. The SLC7A3 promotor region beyond SP1 regulation (CTR) and the β-actin region (ACTB) were used as negative controls; an anti- histone antibody and rabbit IgG were used as assay controls (G). The histogram shows the relative amount of each DNA fragment binding to SP1 (H). The data are presented as the means±SDs. *P < 0.05, **P < 0.01 by one-way ANOVA.

    Journal: Cancer letters

    Article Title: SIRPA enhances osteosarcoma metastasis by stabilizing SP1 and promoting SLC7A3-mediated arginine uptake.

    doi: 10.1016/j.canlet.2023.216412

    Figure Lengend Snippet: Fig. 7. SP1 regulates SLC7A3 expression. A-B. mRNA expression (A) and protein level (B) of SLC7A3 in OS cells with SP1 knockdown or overexpression (n = 3). C. Arginine uptake ratio in OS cells with/without SP1 knockdown upon SLC7A3 overexpression (n = 3). D. Schematic diagram showing possible regulatory sites for SP1 in the SLC7A3 promoter predicted by the JASPAR database (top). Construction of the SLC7A3 luciferase plasmids: wild-type SLC7A3 promoter (WT) and deletion mutants lacking region 1 (ΔRegion 1), region 2 (ΔRegion 2), or both (ΔRegion 1 + 2) (bottom). E. Homology analysis of region 1 and region 2 in the SLC7A3 promoter. F. 143B cells with/without SIRPA knockdown were transfected with plasmids encoding SP1(-500/0)-Luc WT, ΔRegion1, ΔRegion2, and ΔRegion1+2. Then, luciferase levels were measured in extracts after 48 h. G-H. ChIP assay of DNA fragments containing putative region 1 and region 2 within the SLC7A3 promoter region followed by PCR. The SLC7A3 promotor region beyond SP1 regulation (CTR) and the β-actin region (ACTB) were used as negative controls; an anti- histone antibody and rabbit IgG were used as assay controls (G). The histogram shows the relative amount of each DNA fragment binding to SP1 (H). The data are presented as the means±SDs. *P < 0.05, **P < 0.01 by one-way ANOVA.

    Article Snippet: The membranes were then incubated with diluted (1:1000) primary antibodies against human ACTB (4970; Cell Signaling Technology), SIRPA (47027; Cell Signaling Technology), SLC7A3 (PA5-103721; Invitrogen), RNF4 (12187; Cell Signaling Technology), ubiquitin (3936; Cell Signaling Technology), SP1 (9389; Cell Signaling Technology), p-Thr278-SP1 (PA5-106039; Invitrogen), p-Thr739-SP1 (PA5-104771; Invitrogen), ERK (4695; Cell Signaling Technology), p-ERK (9101; Cell Signaling Technology), AKT (4691; Cell Signaling Technology), p-AKT (4060; Cell Signaling Technology), β-catenin (8480; Cell Signaling Technology), and p-β-catenin (9567; Cell Signaling Technology).

    Techniques: Expressing, Knockdown, Over Expression, Luciferase, Transfection, Binding Assay